ISOLUTE SLE Supported Liquid Extraction Products ISOLUTE SLE plates and columns are used for the supported liquid extraction of a diverse range of analytes from aqueous samples such as biological fluids including whole blood, plasma, serum, urine and oral fluid, using a simple load wait elute procedure.ISOLUTE SLE products are easy to use, perfectly suited to automated procedures, and column and plate formats are available for extraction of sample volumes from 1.L to 1. 0 m. L. See our new 4.Jt_jbfsuq.jpg' alt='Is Benzoylecgonine An Active Metabolite Of Fentanyl' title='Is Benzoylecgonine An Active Metabolite Of Fentanyl' />L sample volumes.High analyte recoveries are obtained without any offline steps such as protein precipitation, and sample extracts are free of proteins and phospholipids.Features. Protein and phospholipid free extracts.High analyte recovery with no emulsion formation.Drug+Major+Cmpds.+Minor+Cmpds.+Codeine.+Morphine.+Dihydrocodeine.+Hydrocodone.+Hydromorphone..jpg' alt='Is Benzoylecgonine An Active Metabolite Of Fentanyl' title='Is Benzoylecgonine An Active Metabolite Of Fentanyl' />Fast load wait elute procedure. Application Lifecycle Management Software Wikipedia . Easy to automate for plates and columns.Supported Liquid Extraction.The Supported Liquid Extraction SLE process is analogous to traditional liquid liquid extraction LLE and utilizes the same water immiscible solvent systems for analyte extraction.Instead of shaking the two immiscible phases together, in SLE, the aqueous sample is immobilised on an inert support, and the organic phase flows through the support, eliminating problems such as emulsion formation, and low analyte recoveries.Processing conditions.Processing ISOLUTE SLE columns, 4.Both manual and automated, vacuum or pressure systems can be used.Clinical Interpretation of Urine Drug Tests What Clinicians Need to Know About Urine Drug Screens.Drug screening includes drug tests for up to ten classes of drugs including amphetamine, methamaphetamine, opiates, PCP, cocaine and marijuana.Benzoylecgonine, the metabolite of cocaine, is a zwitterion and cannot be analyzed directly by GCMS after extraction from the urine specimen.VA Academic Detailing Service Real Provider Resources Real Patient Results Your Partner In Enhancing Veteran Health Outcomes VA Academic Detailing Service Email Group.Protein and phospholipid free extracts.Liquid liquid extraction can give particularly clean extracts of biological fluids, as matrix components such as proteins, phospholipids and salts are not soluble in typical LLE solvents, and are excluded from the final extract.Because the same water immiscible solvents are used in SLE, proteins and phospholipids are efficiently removed from the final extract, and no additional steps such as protein precipitation are required.Volume guidelines.Unlike SPE, where the sample is loaded onto the column and flows through the sorbent to waste, in SLE the ENTIRE sample volume is absorbed onto the extraction bed.It is therefore vital to use a format with sufficient capacity to absorb the whole sample volume.Load volume is included in each product description.Table 1 Maximum sample and elution volumes for ISOLUTE SLE products.Product capacity.Maximum load volume.Elution protocol volume.L 9. 6 well plate.L1 x 1 m. L4. 00 L 9.L2 x 9. 00 L1 m. L 4.L5 x 1 m. L2. 00 L Array columnplate.L2 x 5. 00 L4. 00 L Array columnplate.L3 x 7. 50 L4. 00 L column.L2 x 9. 00 L1 m. L column.L2 x 2. L2 m. L column.L2 x 5 m. L5 m. L column.L3 x 8 m. L1. 0 m.L column. 10 m. L2 x 2.LComprehensive User Guide.The ISOLUTE SLE User Guide describes how to develop supported liquid extraction methods using ISOLUTE SLE products, along with hints and tips for optimising performance and extending the range that can be extracted.Applications. A number of application notes are available describing the use of ISOLUTE SLE in the extraction of therapeutic drugs, drugs of abuse and their metabolites, vitamins, steroid hormones and others.
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